Using A375 to stimulate NK cells to release CD107a and IFN - γ in a 1:1 ratio, but the experimental results show that NK cells have a lower ability to release CD107a and IFN-γ under A375 stimulation. How should I change the ratio of NK cells co cultured with A375 cells to evaluate the expression of CD107a and IFN-γ in NK cells? Should more target cells be inserted? In what proportion?
Hameer Khan Khaskheli
To increase CD107a and IFN-γ release, set the E ratio to 5:1 or 10:1, resulting in more NK cells per A375 target. Higher E ratios often increase NK cell activation and cytokine release.
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