I need the simplest method of detecting vitamin A,B,C,D and E in a filtered sample apart from HPLC. Thanks
As a mixture, no. They interfere with each other. Use a more selective technique such as HPLC for higher quality results.
You can use spectrophotometry to detect vitamins A, B, C, D, and E, utilizing specific reagents that form colored complexes for measurement. Other methods include high-performance liquid chromatography (HPLC), fluorescence spectroscopy, and electrochemical techniques, which offer varying sensitivity and specificity. Spectrophotometry is a cost-effective and rapid option for vitamin analysis.
Dear 4d ago
Nelson Okon,
This requires method development. Kindly follow the steps for method development.
1) Scan individual Vitamin in the range of 200nm to 800nm.
2) Find out the wavelength of each individual vitamins. I just checked on google and Vitamin A is showing 325nm (Visible range), Vitamin B is 210 (UV range) Vitamin C & E showing 245nm (UV-range), vitamin D 280 to 315 nm ( UV & Visible range both).
3) Though I have provide you wavelength of all vitamins but scanning needs to be done once. This is first step of method development.
4) check the absorbance by preparing any concentration so that absorbance should be between 0.60 to 1.20.
5) As per solubility prepare samples and scan. Record the absorbance and calculate against standard absorbance.
6) Validate the method
7) After validation, method can be used.
Hopefully I have provided procedure in details
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