Are they any methods of purifying sub 100 base pair ssDNA sequences directly from gel electrophoresis?

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Rajesh Dev Sarkar

Better to use Acrylamide gels.

You can have a look at this earlier discussion-

https://www.researchgate.net/post/good_protocol_for_purifying_single_strand_DNA_through_gel_electrophoresis/amp

Simon Butler

Thank you Rajesh Dev Sarkar

António Maximiano Fernandes

Yes is possible. But 100bp is to small and special care should be taken. Long and forced centrifugation may lost your dna thorough filter