Is any way or method to seal agarose pad or channels fabricated agarose gel pad to glass slides as like we do plasma bonding with PDMS to slides?

Is any way or method to seal agarose pad or channels fabricated agarose gel pad to glass slides as like we do plasma bonding with PDMS to glass slides?.

04 January 2021 3,589 2 View

Comparative STUDY: sample of sector A in 5 cities and sector B in whole country for ?

sector A sample is 313, and sector B sample is 200. If I take sector B population in the whole country then sample B's comes = 330 Q. Can I take sector A in 5 cities and sector B in the whole...

27 December 2020 6,930 2 View

Find value of heat transfer coefficient in SI units??

A tube is subjected to air at standard temperature and pressure conditions with velocity 5 m/s. initial temperature of the tube is 980 K and drops to 500 K after interaction with the cool air....

21 December 2020 6,408 3 View

Can we transform any gfp tagged plasmid vector in to any E.coli strains with a objective to visualize E.coli bacteria as fluorescence molecule?

Can we transform any gfp tagged plasmid vector in to any E.coli strains with a objective to visualize E.coli bacteria as fluorescence molecule?. I need a valuable suggestions so Please help me....

30 November 2020 2,469 8 View

No title

23 November 2020 6,277 5 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

How can we define/simulate shell elements (S3/S3R, S4, S4R, SAX1, SAX2) in ABAQUS using a python script while defining bending behavior of composites?

Working with ABAQUS 6.14 for defining the UMAT subroutine for the initial imperfection of shell elements.

19 October 2020 974 1 View

How to approach Emotiv Epoc results and analyze them for results and significance for a study linking education and neuroscience?

Hey all, I am currently a master's student at Queen's University in Canada pursuing in the field of education. My informal thesis idea is to inform education experts through the use of...

02 October 2020 5,030 1 View

How can I couple x-coordinates with y-z coordinates in COMSOL?

Hi, I want to couple x-coordinates with y-z coordinates so that the shape of the whole plate looks same movement. For example, if cross-section of the plate i.e. y-z axis shows deflection then...

25 September 2020 5,941 3 View

Hey, How can I implement Kernal Extreme Learning Machine?

actually I want to develop a system which predicts the accident severity using CNN for feature extraction and Mixture of experts including a variation of ELM Extreme Learning Machine (KELM, CELM,...

18 September 2020 2,011 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View