How to determine number concentration of polymer solutions using viscosity experiment?

I have synthesized PNIPAM (Poly (N-isopropylacrylamide)) microgels, which are copolymerized with oligo-ethylene glycol based comonomers. I have to determine the exact number concentration for each...

19 February 2021 5,205 5 View

Could anyone explain me this CD graph?

There is a strong negative peak at 228 nm. What can we conclude about secondary structure of this protein?

08 February 2017 2,788 5 View

Protocol for indirect elisa quantification of cytokines (IL-10, IFN-gamma) in serum/plasma?

22 January 2017 8,979 3 View

I am looking for oral cancer cell lines. Can anyone provide proper information?

hii everyone,I have to initiate my work on oral cancer cell lines but unfortunately I am not able to get it any where in India. I have contacted NCCS (Pune) as well for the same concern but they...

20 October 2015 3,497 3 View

While doing CFD analysis using Fluent for unsteady 2D flow past cylinder, I'm getting very low values of lift coefficient; how do I correct it?

04 October 2015 139 6 View

Why is the velocity of X-rays in glass and in other materials greater than its velocity in empty space?

Why is the refractive index for X-ray less than unity?

26 March 2014 4,842 34 View

How to prevent macro segregation in medium carbon steel? Please suggest Paper>>>

How to prevent macro segregation in medium carbon steel..

01 January 1970 7,181 5 View

How to calculate excess CO2 mathimatically in Electric arc furnace? And Utilization of Excess CO2 in Electric arc furnace?

To Find the optimum combination of Oxygen and coke flow rate during lancing and to minimize tap to tap time in EAF. 1- Determination of Scrap Mix to be used in EAF, Control in Met coke feed rate,...

01 January 1970 5,579 2 View

Why bursting defect come in SCR420HB grade, during rolling?

01 January 1970 3,142 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View