Has anyone had problem with single cell whole genome amplification?

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whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?

07 September 2020 7,431 5 View

A question regarding Response factor in GC. what does area count mean?

i was reading about response factors in GC and i encountered the term area count, I was wondering if anyone can explain it to me, i have a general idea about the meaning but i'm not sure. does...

30 March 2020 7,854 1 View

Which software is best for estimating of pipeline corrosion rate?

I want to calculate Steel pipeline corrosion rate in different conditions, so Which software is best for estimating of pipeline corrosion rate?

03 March 2020 1,779 4 View

EEG connectivity calculation via HERMES toolbox ?

Hello everyone I am currently trying to calculate phase lock value for my EEG data with the help of the HERMES toolbox in Matlab. I have a total of 48 subjects with different channel location...

04 January 2020 5,220 1 View

How can one simulate agglomerated cork in ansys and which engineering model can provide the better results?

I'm going to simulate the structural response of a sandwich structure with agglomerated cork core under bending and compression loading. Is there any reliable information about the structural...

28 August 2019 2,843 2 View

Hi.I have an extruder for dewatering a two phase fluid. I want to setup slip condotion in wall boundary condition in Ansys Fluent. how can I do that?

in wall boundary dial box I should specify the shear stress in three direction (x,y,z). how can I measure this shear stress? please help me....

10 June 2019 7,550 1 View

What are the best polynomial approximation functions for analyzing free vibration and impact analysis of plates?

I want to do a free vibration analysis for a plate. In this research, I want to use the Ritz method. For Ritz-shape functions, which are the best polynomial functions that give the most accurate...

10 April 2019 2,612 5 View

Can I still publish a paper if there is something rather similar in a patent?

I've tested a variety of reagents for the synthesis of a compound and found one that has never been used before and yields a good product. after my experiments i did some research and came across...

04 September 2018 5,726 3 View

What does a higher (or lower) soil sorptivity indicate?

sorptivity is defined as the capacity of soil to absorb or desorb water by capillarity. but what does higher sorptivity mean? does it mean the part of infiltartion that depends on capillarity...

30 July 2018 4,467 4 View

A question about MALDI/TOF MS?

I am a Ph.D. student of microbiology in Iran. Actually, I have found a bacterial peptide that I want to identify that using MALDI TOF MS. I am looking for anyone with experience with MALDI TOF MS...

23 April 2018 9,654 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View