Hi,

To separate try a gel filtration with a colum such as superose 6.

To characterize your proteins, I will work with analytical ultracentrifugation (velocity sedimentation).

Best,

Sébastien

I think you can use Tricine—Sodium Dodecyl Sulfate—Polyacrylamide Gel Electrophoresis

you could try isoelectric focussing which works on separating different charged proteins in either liquid ( old fashioned) or inert matrix so the protein moves to its isoelectric point and then stops moving. Movement can be around the gel matrix particles rather than through it