How can I calculate the sample size required for evaluating a novel immunohistochemistry (IHC) antibody?

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I am getting command line parse error; inknown option conf?

I have tried the solutions provided in the answers by other researchers, but I still can't figure it out. I am a newbie to this field. Please help m ekindly. I have attached the conf.txt file...

17 April 2024 357 3 View

How artificial intelligence (AI) contribute to the sustainability of the green products?

After the great development in information technologies, it has become necessary to know the contribution to the development of green products

29 August 2023 2,655 4 View

How did traumatic situations from war experiences lead to a chemical mark on a person’s gene, which can be passed down to future generations?

Intergenerational trauma

30 May 2023 4,187 1 View

How to plot this simple step pressure response of an integral controller using SIMULINK?

I don't know how to use SIMULINK... Also, the science of control systems is my Minor field of research ... I want to enhance my skills for this field.. Help me to plot and estimate the...

06 February 2020 8,658 16 View

Do you have any application of thermal engineering that is solved using SIMULINK?

I'm searching for MATLAB/SIMULINK tutorials that predict the temperature or pressure response of any negative feed back closed loop control systems such as: 1- Temperature control of fluid in...

08 December 2019 3,465 5 View

What is the best Simulink text book that is most suitable to solve applications of thermal engineering?

Hi.. What is the best MATLAB text book that is most suitable and full of solved examples to teach students how to solve applications of thermal engineering using Simulink?

05 December 2019 8,457 2 View

What is the best time point to detect IL-6 using ELISA?

Hi, I prepared cell culture from mice lymph nodes. I did ELISA for IL6; however, the levels of IL6 were really low to detect at 48hrs, 72hrs, and 96hrs. I have read in the literature that to...

13 February 2017 774 4 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View