Our AKTA Explorer FPLC computer hard drive just failed and we do not have access to the Unicorn 5.31 software to run the FPLC. Does anyone have a disc of that version of Unicorn workstation software that we could buy or borrow?
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...
02 March 2021 1,830 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...
01 March 2021 4,128 3 View
Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...
01 March 2021 9,015 3 View
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.
28 February 2021 3,640 3 View
The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...
28 February 2021 6,560 14 View
I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...
27 February 2021 6,818 3 View
Hello all, I frequently use the Akta purifier for protein purification and recently have been getting error messages in unicorn saying "error 2012 no response frac, 900". The frac 920 is designed...
02 September 2020 2,757 1 View
Recently, my professor has given me a task to learn how to operate the FPLC system. I plan to run protein samples either HIC or IMAC column. I have ran tiny spin columns in the past, so I know how...
02 March 2018 7,958 4 View
We've tried powering off the AKTA system, as well as shutting down the PC, but our CU-950 is unable to connect to the AKTA system for us to connect using the Unicorn software. The CU-950 indicates...
28 November 2017 2,037 3 View
Akta Pure limits the number of columns per system to 5 due to the C-valve. What is the best way to add more columns to the system? I've heard of people using the loop valves to double the columns...
21 November 2017 6,224 3 View
10 November 2017 3,035 2 View
10 November 2017 2,623 3 View
Dear Colleagues, Should you explain the existence of fishes and other sea creatures in closed lakes that don't connect to any river, sea or ocean. The resource of these lakes is only sky rains?...
06 July 2017 4,397 1 View
For my research, I must use AKTA protein purifier and UNICORN software but I don't understand them. How do you use them? Can you help me ? Do you have a method or a protocol ? Thanks in advance....
02 March 2014 2,098 4 View