On every gel of this type that I've ran so far, I'm getting this large band interfering with my gel analysis no matter how much troubleshooting I've done. This is a Novex Wedgewell 6% Tris-Glycine Gel and in each well I've loaded 10ul of the Novex 2X Native Tris-Glycine Sample Buffer + 10ul of my sample. I ran the gel at 100V for 1hr at 4C.
I've troubleshooted the gel by pre-running it and also trying to load without any 2x loading buffer, but the band has still remained.
Michael J. Benedik
The problem is not what you loaded into the wells since it spans the entire gel. It looks to me like something in the upper tank buffer is causing this dark band.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Physical Phenomena
- Luminescence
- Fluorescence
More Shashwat Limbasia's questions See All
Similar questions and discussions