Hello, My colleagues provided me with a protocol for MCF10A media, but it substitutes cholera toxin with forskolin. Will this modification be effective?
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,167 1 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 477 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
I am working on MCF10a cells. I started my culture by thawing a fresh vial of cells purchase in the month of February, 2024. I am culturing them in DMEM/F12 media supplemented with horse serum,...
10 March 2024 8,581 2 View
During preadiopcyte in vitro differentiation, cocktail stimuli is needed. But why IBMX but not forskolin? Both of them can increase the intracellular level of cAMP and lead to PKA pathway...
28 February 2024 8,146 0 View
Most of the commercial antibodies available seem to target the T18 domain (aa 225-339) of the adenylate cyclase, or they do not specify which domain. I am in search of an antibody specific to the...
18 December 2023 158 0 View
Hi Everyone, I am optimizing cAMP HTRF assay for the CCR2 receptor which couples to Gi. I wanted to know what is normally used and if you have experience in what works better for you. Adding the...
15 August 2023 7,653 1 View
Would anyone know how to answer this question? Or let me know which supplements are really needed at this stage of building the 3D skin?
01 March 2023 2,903 1 View
Hello Scientists! I’m trying to resuspend CTB-Alexa 555 from Invitrogen (C34776) as manufacturer recommend (In PBS, pH 7.4). But I found a several precipitates as the final result. Does anyone...
27 February 2023 6,675 0 View
Dear fellow olfactory scientists and/or electrophysiologists, For one of my projects, I would like to stimulate/activate different primary olfactory transduction cascade stations. As a first...
16 January 2023 8,014 0 View
I run a qPCR (Taqman probe) to detect shiga-toxin gene (stx1) with rotar gene Q (qiagen) thermal cycler. My primer & probe are - F - 5'-TTTGTYACTGTSACAGCWGAAGCYTTACG-3' Base - 29, GC - 44.8%,...
17 December 2022 2,981 3 View
I recently tried to induce myocarditis in BALB/c mice using the following protocol: Day 0: Subcutaneously injected 200 ug of MyHC614-229 peptide (Ac-RSLKLMATLFSTYASADR-OH) emulsified in complete...
05 December 2022 7,015 1 View
Literature suggest avoid co-administration of Cholera and yellow fever vaccine and there should be minimum gap of 3 weeks between them . What may be the reasons?
11 June 2022 3,509 3 View