I would like to run the FAM labelled oligo DNA on a agarose gel and detect it with Biorad Chemidoc MP, it has "Nucleic acid gels" option, "Protein Gels" and "Blots" Fluorescein channel. Which should I choose as my gel is not stained with anything but only the DNA is labelled with FAM, I wonder if I should still choose nucleic acid gels option.
Stanley Ng
FAM needs the Fluorescein channel, the system will use the blue LED excite the FAM to cause the emission of green light.
If you use the nucleic acid option, the system will use the UV to illuminate and you'll see not a lot....
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Leonardo Tejo Gunawan
Emily Tsui You can choose Nucleic Acid application and pick SYBR, because the FAM molecule and SYBR have a similar ext/emm wavelength.
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