Is there any alternative option in culturing MCF-10A without using cholera toxin? Can I use Lonza MEGM bullet kit(cat no. CC-3150)? Someone having the expertise in culturing MCF-10A, please guide me.
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12 June 2024 1,244 2 View
One of my scholars is about to complete a thesis on mindfulness, resilience, and wellbeing. This university requires thesis examiners from different parts of the globe. Should be an associate...
02 June 2024 860 1 View
Greetings, despite MIMO and array fall under the umbrella of multi antenna system, what are the features that distinct one from the other. At the same time, what factors makes one to be far...
16 April 2024 9,905 2 View
Greetings, what literally governs the metamaterial to render epsilon negative or mu negative phenomena. Is there any theory behind finding the same by looking at the structural design of...
31 March 2024 7,539 3 View
Greetings, while realizing the circuits for microstrip patch antenna, which component contributes more towards controlling the bandwidth.....
26 March 2024 3,446 0 View
Greetings, what literally makes the simulation results of same model in CST and its equivalent circuit counter part in ADS ends up with distinct results. Say the S21 value of a model in CST is...
21 March 2024 1,090 4 View
Greetings, in both spatial multiplexing and beamsteering we expect significant beams to emanate from the dedicated radiator to render its service. Then what makes them distinct.
21 March 2024 4,889 0 View
Greetings, come across art literatures on broadband metamaterial absorbers (BMAs). BMAs despite its simple structure they render absorption of em waves for broad range of frequencies. My query...
14 March 2024 5,983 2 View
Greetings, in continuation to the query raised the answers on the same is awaited. If possible supporting the same with necessary specification sheets too.
14 March 2024 6,097 0 View
Greetings, we all know that apart from defected ground structure there are a lot more way to address coupling between the radiators in MIMO antenna. But making a complete split at ground plane...
20 February 2024 8,389 1 View
I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...
07 July 2024 9,034 2 View
Usually, basal media have been recommended for the culturing of human epidermal keratinocytes, and most articles suggest growing them in serum-free media. I'm finding it a bit challenging to...
24 June 2024 2,609 3 View
I have been trying to culture P. falciparum, but I see contamination after ~1 week of culturing. What could this organism possibly be (image attached)? The culture media consists of RPMI, HEPES,...
15 June 2024 5,515 5 View
Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...
10 June 2024 4,152 1 View
I'm trying to culture the cell lines H69 and H69AR. Regarding the parental cell line, I'm finding my way in the handling, but with H69AR it's been troublesome. Currently, I'm culturing H69AR in...
05 June 2024 3,471 0 View
Hi Everyone! Currently, I am culturing the BM-MSCs, but it is taking very long, and the yield is very low. I am using DMEM+10% FBS+L-glutamine and I tried the Mesencult media as well. Please let...
02 June 2024 2,140 0 View
I am culturing HEK293 cells. When I plated these cells in a 96-well plate, the cells looked healthy and there were no precipitates. However, after I dosed them with the drug RITA, they developed...
30 May 2024 5,169 2 View
Hello everyone, I would like to culture muscle cells, specially Human Umbilical Aortic Smooth Muscle Cells (HUASMCs) in DMEM. There are various papers out there where this has been done, however,...
28 May 2024 1,398 0 View
Hi everyone, I am culturing colorectal cancer organoids (Control and Experimental) and I observe a faster growing (number and size of organoids) in experimental condition. Now the question is,...
27 May 2024 9,258 0 View
I am having a difficult time finding such a supplement, it's either discontinued by the manufacturer or for bovine or mice.
30 April 2024 6,993 0 View