What standardized protocols are available for measuring the antioxidant enzymes(SOD, CAT and GPx) in zebrafish embryos?

18 September 2024 0 1K Report

Can you kindly share the standardized assay protocols including the reagents, concentrations, reaction conditions, sample preparation and the handling guidelines?

Badges
Science topic

More Sunandita Datta's questions See All

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

How can we deal immediately with a person having anxiety attacks?

there can be some triggering situations, how do you handle them?

18 July 2024 7,076 3 View

What is the degree of crystallinity of a crosslinked polyethylene (PEX-A) pipe? Is there some kind of index? If yes, where can I find them?

I need the crystallinity percentage value of crosslinked polyethylene (PEX-A). I know that they have more than 70% crosslinking. Is there any way to calculate the crystallinity percentage from...

22 April 2024 3,007 0 View

How to check dipole-dipole interaction strength between two molecules when H bonding may not be the stabilising factor in a dimer molecule?

I have optimised the geometry of the dimer of a molecule using pcm solvent model and B3LYP functional with 6311++G(d,p) basis set in Gaussian 09 software. There is possibility of C-H...O H bonding...

01 April 2024 1,820 1 View

How to dissolve Pyrazinamide to administer in mice?

Hi, I intend to give mice 150 mg/kg of pyrazinamide. I need 3.7mg per mice and 100ul needs to be administered. The drug only dissolves in DMSO and the final amount of the DMSO in water exceeds...

27 September 2023 6,416 0 View

Without measuring lifetime, how to distinguish between fluorescence and delayed fluorescence?

The PL int will be higher in the case of Fl, but both are supposed to overlap. Then how do know if the system has delayed Fl emission?

05 September 2023 3,068 4 View

How a flame within deep tube well water in remote village can be explained as well as eliminated ?

Image Ref : The Telegraph Online ( 16.06.18 )/ 19.07.2023.

18 July 2023 397 8 View

What is the difference between PBS and Sodium Phosphate?

Sodium Phosphate manufacture uses sodium dihydrogen phosphate and disodium hydrogen phosphate. PBS manufacture uses pottasium ohosphate monobasic, sodium phosphate dibasic and sodium chloride....

03 April 2023 1,039 2 View

Is anyone using the DiameterJ Plugin to determine the fibers' diameter from SEM images?

Currently, I am trying to determine the fiber diameter in ImageJ using DiameterJ Plugin. Although I created segmented images using the diameterJ plugin, after analysis DiameterJ does not generate...

30 March 2023 9,391 6 View

Is there any solution for memory allocation problem of using SMOTE for imbalance dataset?

I am using SMOTE in a deep learning project for Minority Class Oversampling. But it costs a huge memory and Session Crash in different IDEs. Can anyone give a solution regarding to this issue?

13 March 2023 8,765 3 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

How Can I Apply Hashin Damage Properties to Solid Elements in Abaqus Without Using VUMAT?

Sure, here is the translation: I have a 3D orthogonal woven composite structure where warp, weft, and binder yarns are oriented in three directions. After modeling these yarns and the matrix in...

28 July 2024 8,169 0 View

Will the catalytic effect of retained austenite happens in steels including RA?

The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...

27 July 2024 8,508 0 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View