A small peptide chain was prepared on a solid phase (resin) and stored (after drying) without removing its protecting groups (on the N terminal) for a few months. Later, a deprotection reaction was performed with Piperidine/DMF, and the Kaiser test was performed. However, the test gave a negative result.
Leonardo González Ceballos
-improper swelling of the resin after such a long time standing...
-was the deprotection solution also not used for the same time? That could lead to piperidine oxidation...
-Where the solutions for the Kaiser test also unused during the same time? That´s also a thing to consider...
-peptide decomposition if not stored at least in the fridge...
I hope it helps
Good luck!
;-)
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