Dear all,

Hello, I am refining a protein structure obtained from X-ray diffraction.

It is ~ 300 aa long but there are about 7-8 consecutive amino acids in the middle with weak electron density.

I was trying to fit the amino acids with their side chains by lowering the rmsd but it shows high RSRZ value as high as 4~5.

In addition, there are ~ 18 RSRZ outliers which is 6% of total.

So I thought if I remove those consecutive amino acids or their side chains, the statistics would look okay since I fixed all Ramachandran outliers and Rotamer outliers already.

Please let me know if you need more details to figure this problem. I will upload some more.

Thank you in advance!