For qualitative analysis of gram-negative bacterial biofilm. What is the ideal stain that we can go with? I have tried CV, it gives good images but not good compared to gram-positive ones. It gets washed during washing steps. On searching I got Safranin and other alternatives. If anyone has used this. Please share your experience
Pseudomonas aeruginosa would be a good option.
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@Priyanka Singh
For gram negative biofilm the prefered stains are fluorescent dyes like SYTO 9 and DAPI, as they can penetrate the biofilm matrix and bind to the bacterial DNA
Rabia Ikram apart from fluroscent dyes...for simple light microscopy
@Priyanka Singh
For simple microscopy there is Crystal Violet Gram used .
If your expectation is to qualitatively observe biofilm formation congo red is ideal
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