How do I successfully culture primary human leukocyte from TLRE filters and improve viability of the cells in suspension?

17 June 2024 0 4K Report

I am currently culturing leukocytes from TLRE filters from our blood bank. I have been struggling to culture these cells, as well as improve their viability. I was wondering if anyone would be able to share their labs protocol on how to recover cells from the TLRE filters and any other information that could help our lab learn to handle cells in suspension.

Thank you!

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