I have cell suspension which I need to store in RNA later. How much volume of RNA later should I use? And at what temperature and duration can I store the cell suspension in RNA later?
Please read the manufacturer’s instructions
https://www.thermofisher.com/content/dam/LifeTech/migration/en/filelibrary/nucleic-acid-purification-analysis/pdfs.par.18819.file.dat/bp-7020.pdf
You must store it at -80 Celsius degree and in a proper medium of suspension. TA or TAE sterilized.
• Most samples in RNAlater Solution can be stored at room temperature for 1 week without compromising RNA quality, or at –20°C or –80°C indefinitely.
• Do not freeze samples in RNAlater Solution immediately; store at 4°C overnight (to allow the solution to thoroughly penetrate the tissue), remove supernatant, then move to –20°C or –80°C for long-term storage.
Rna later could be store your Rna at -80 C for several years 2 or 3 as minimum.
- Badges
- Science topic
- Similar topics
- Agricultural Science
- Agronomy
- Crop Science