I am trying to establish a cell line based xenograft model of CML in SCID mice. I tried to inject 5x10^5 K562 cells (in saline for injection) but there was considerable difficulty in injecting the cells via the tail vein (used an insulin syringe). There was mainly back flow of the cells. I tried in another animal, but this had gone in subcutaneously.
Can anyone suggest any specific method they have used to deliver cells via the tail vein?
We do commercial K562 xenografts (http://altogenlabs.com/xenograft-models/leukemia-xenograft-model/k562-xenograft-model/) with subcutaneous injections into the hind flank consisting of one million cells in matrigel. Tail vain injections are generally easier with matrigel, and the solidification upon injection helps prevent backflow.
Dear Lakshmy
I am sending you one of our papers were we do tail vein injection. We do so a lot.
http://ar.iiarjournals.org/content/34/9/4761.long
Couple of things: be sure to put the tail of the animals in warm water before injection, that will expand the vein injection. However, it takes a lot of practice. It takes a lot of time for an student to get to manage the technique. Therefore, keep doing it.
Good luck!
Daniel
We do commercial K562 xenografts (http://altogenlabs.com/xenograft-models/leukemia-xenograft-model/k562-xenograft-model/) with subcutaneous injections into the hind flank consisting of one million cells in matrigel. Tail vain injections are generally easier with matrigel, and the solidification upon injection helps prevent backflow.
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