Can anyone recommend a good protocol for C.elegans spike in for isolated miRNA from tissues?

Siti Maizatul Syafinaz Mohd Shahadin @Siti-Maizatul-Syafinaz-Mohd-Shahadin

10 August 2017 1 9K Report

I have an isolated RNA, extracted from human tissues biopsy. I want to determine the expression of miRNA by qPCR by using miScript miRNA PCR Array by Qiagen, and C. elegans spike-in is used as one of the normalization control. Thus, I need to add C. elegans spike-in during cDNA synthesis protocol. I am looking for any suggestions or insights, particularly if someone has done this before.

Najat Abdulrazzaq Hasan

you can follow the instruction of the Exiqon company for the spike in kit:

https://www.exiqon.com/rna-spike-in-kit

good luck

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