Dear all,
Does anyone know a publication/ data in plants or yeast on the proportions of DNA repair via NHEJ and HR in different stages of the cell cycle comparable to the findings of Mao et al. from 2008 (DNA Repair 7: 1765-1771 and Cell Cycle 7(18): 2902-2906) in mammalian cells?
Contrary to the common hypothesis (NHEJ in G1 phase, HR in S and G2) their studies show NHEJ as very fast and predominant repair pathway throughout the cell cycle including S and G2, the highest HR rate was measured in S-phase (but still much less than NHEJ). I would be interested to lean about similar studies in yeast or plants.
Thanks a lot!
Gertrud
To my knowledge, no such detailed measurements are available for plants. You may ask Holger Puchta about this.
Best
This might be useful as a starting guide.
https://www.ncbi.nlm.nih.gov/pubmed/17072889
May be this book will give you guidance
Phytosfere'99 - Highlights in European Plant Biotechnology Research ...
https://books.google.co.in/books?isbn=0080538991
G.E. de Vries, K. Metzlaff - 2000 - Business & Economics
In plants, gene targeting frequencies, expressed as the ratio of the number of ... Transgene integration exploits pathway repairing DNA double-strand breaks ( DSBs). ... In yeast, HR is the predominant process and mutations in components of HR ... NHEJ repairs DSBs by joining the two ends of a brokenDNA through a ...
It seems counter-intuitive but I'm afraid I have no experience in plant DNA repair. Certainly in mammalian cells NHEJ is suppressed during S-phase when HR takes over. Look carefully at the data
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