How to determine the concentration of an inhibitor?

06 June 2017 1 1K Report

I want to calculate the IC50 of a inhibitor compound. The inhibitor was obtained through insilico approaches. Now I need to perform the validation study for the inhibitor. To perform the assay I know the enzyme and substrate concentration but i don't know how to set the inhibitor concentration range (either in nM or in mM).

Also, I would like to know how many concentrations should I use?

Sathya B

Thank you for your reply, sir.

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