I am looking for something like NCI's TCGA databases (with patient county / area).
I know any private / personal identifying information is removed but if anyone has an idea, please let me know!
Thank you
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Hello, I'm newbie to TCAD. I wanna calculate Breakdown Voltage of AlGaN/GaN HEMT. But, my code shows just saturation. Could you please help me how to simulate Breakdown Voltage of my code? And...
25 July 2024 3,738 1 View
Is it the best optimization ratio based on experiments?
22 July 2024 8,170 0 View
Hi everyone, I'm working on calculating the activation energies for some redox reactions using Gaussian, Here are the reactions I'm interesting: Py•−+ 3O2 → Py + 3O2•− Py•− + 1O2 → Py + 1O2•− Is...
18 July 2024 4,418 3 View
I am a master student and writing research paper. I have 3 yes/no questions, 2 multiple responses questions and 1 Likert scale question. I would like find out whether age has relationship to my...
05 June 2024 1,564 9 View
Can the OH of hydrogen sulfate also react with isocyanate to form a urethane bond?
07 April 2024 7,739 0 View
While reading papers on polyurethane synthesis, a question arose. Q1. MDI [methylene di(p-phenyl) isocyanate] 2mmol PTMO[poly(tetramethylene oxide)] 1mmol 1,4-butanediol 0.9mmol...
27 March 2024 2,718 2 View
In linear polyurethane, which is made by reacting isocyanate with two NCO and -diol with two OH, the R ratio (the ratio of NCO to OH), cannot theoretically deviate significantly from 1:1, right?
25 March 2024 4,488 8 View
It is said that dodecyl-trimethylammonium chloride (DTA) is used to dissolve hyaluronic acid (HA) in an organic solvent such as DMF. If the experiment is conducted using the method below, will...
23 March 2024 4,395 4 View
When making medical TPU using PCL, some papers use stannous octoate, while others do not. Is stannous octoate really necessary?
18 March 2024 8,587 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...
05 August 2024 8,025 7 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View