Especially isolation source, host, and environment etc,... with emphasis on Rhizobia if applicable.
JGI/IMG
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
i want to know; that how to proceed with the mentioned data set to capture the long run and short run association in panel data, thank you
15 May 2024 6,946 6 View
We are looking for a research group that contains a research group in the field of management and human resources?
16 February 2024 9,102 6 View
Aslamo alikom/ Greetings everyone, I'm conducting a western blot experiment (8% SDS gel) and I wanna test 2 proteins, one is 95 KDa, and another is 35KDa. The antibodies I've for both are mouse...
25 January 2024 7,638 2 View
Hello/Salam Everyone, I'm using GraphPad prism to calculate the IC50 of some drugs in cancer cell line and impact of drug B on the IC50s of these drugs. As per my calculation method, to conduct...
20 January 2024 7,527 0 View
Aslamo Alikom/Greetings everyone, I've been working on MCF7 cancer cell line, and I would like to study their response to cyclophosphamide in vitro. I was wondering what's the best solvent to...
08 November 2023 3,712 1 View
Dear: I would like to prepare a sterile solution for injection of animals under my experiment with 1,3-ᴅ-glucan. but it was recorded that, steam autoclave affects the criteria of the extract. So,...
24 September 2023 430 0 View
I got two conferences and i think of higher acceptance rate, but I am afraid to withdraw and lose the time already taken for review.
24 July 2023 795 4 View
for my experiment i want to know How to calculate the multi class experiment sample number with Gpower?
10 July 2023 208 0 View
I've been working on MCF7 cell lines for nearly 7 months now. Today while checking one of the flasks, I found its media color dark green and all cells floating dead. I've trypsinized this flask...
23 May 2023 1,374 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...
20 July 2024 1,339 2 View