1. I have used anti bacterial & anti mycotic [ pencillin streptomycin & amphotericin B ] at required concentration still I face contaminations , so is there any other way out to prevent contamination
Saif Wahid
Proper aseptic technique: Ensure that you follow strict aseptic techniques during sample collection, handling, and culture preparation. This includes thorough handwashing, wearing sterile gloves, and working in a clean and controlled environment such as a laminar flow hood or a biosafety cabinet. Surface disinfection: Clean and disinfect the surfaces and equipment you will be using for sample collection and culture preparation. Use appropriate disinfectants such as 70% ethanol or a suitable alternative to eliminate potential contaminants. Sample collection: Ensure that the collection tools, such as swabs or brushes, are sterile and specifically designed for the type of sample you are collecting. Use aseptic technique during sample collection to minimize external contamination. Culture media: Use commercially available sterile culture media or prepare your own media using sterile techniques. Sterilize the media by autoclaving or filtration, depending on the specific requirements of the media. Incubation conditions: Set up appropriate incubation conditions for the culture, including temperature, humidity, and gas composition. These conditions should favor the growth of the desired microorganisms while inhibiting the growth of potential contaminants. Contamination monitoring: Regularly monitor the cultures for signs of contamination, such as changes in color, texture, or the presence of unexpected microbial growth. If contamination is detected, it is essential to discard the contaminated culture and start again with a new sample. Antibiotics and antifungal agents: As you mentioned, you have already used antibiotics and antifungal agents like penicillin, streptomycin, and amphotericin B. It's important to ensure that these antimicrobial agents are effective against the specific contaminants you are encountering. Consult with a microbiologist or refer to the literature to determine the appropriate concentrations and specific agents for your target microorganisms. Periodic maintenance and cleaning: Regularly clean and maintain your equipment and laboratory space to minimize the chances of contamination. This includes disinfecting work surfaces, sterilizing reusable equipment, and properly disposing of waste materials.
Contamination in oral samples for primary culture can be a common challenge, but there are several additional steps you can take to minimize the risk of contamination:
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