I need to know the types of RNA that you can analyze in an electrophoresis gel and the coefficient of sedimentation of Arabidopsis thaliana and CHO cells.
I don't find the answer on the internet.
Thank you :)
Ok, I see. Thanks a lot for your help, I really appreciate it.
I was asking because in my manual I have to fill this table and I don`t know what information I have to put. If you notice the part of E.coli, there is specific information, that´s what I´m looking for but I can´t find it. Do you know how can I search it or find the answer?
@ Ali,
CHO should be the Chinese hamster ovary (CHO).
GHO cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of therapeutic proteins
@ Michel,
Please see some examples of the rRNA sizes (Kb) of different species at link below:
[https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rna-isolation/general-articles/ribosomal-rna-sizes.html]
Human: 18S, 28S
Mouse: 18S, 28S
Xenopus: 18S, 28S
Drosophila: 18S, 28S
Tobacco Leaf: 16S, 18S, 23S and 25S
Yeast: 18S, 26S
E. coli: 16S, 23S
@Yuan-Yeu Yau
Thank you for your help, It´s so useful. You give me another idea to find the answer to my question. When I finally find it I will give you the answer, in order to complement our knowledge.
Thanks a lot.
Like human and mouse, Chinese hamster is a mammal. So, I think they should also have the rRNA with 18S and 28S.
Attached Figure shows tobacco rRNA on a gel. From: Zymo Research
The legend of the Figure:
"Isolation of total RNA from 10 mg of a fresh leaf material (Nicotiana sp.) using the ZR Plant RNA MiniPrep™. Leaves were minced then processed using a FastPrep®-24 instrument (MP Biomedicals). Samples 1 and 2 were loaded in 2x and 1x volume aliquots, respectively, and resolved in a 1% (w/v) nondenaturing agarose gel. RNA Millenium™ Markers (Ambion) were used as size standards."
http://www.zymoresearch.com/rna/total-rna-purification/soil-fecal-plant-rna/zr-plant-rna-miniprep
@ Ali,
For a rookie or a beginner sometimes it is hard for them to find a way to get started for a question (because they have no experimental experience at all). For giving them a few detail answers will help them in figuring out how to approach this kind of question later (and we ourselves also learn from doing this, too). For example, one of the answers provided above can give them an idea that the rRNA patterns on a gel can be different from different organisms. Also the gels used to run can be 'denatured' or 'non-denatured', what does that mean. The attached Figures show some stuffs that are not asked in his lab notebook. And I believe they will go online to find more related stuff if they are interested..
Besides, those answers are not only for the person who post this questions, but also for others who are following or read this question.
But, thank you for your suggestion.
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