Hello.
I'm recently researching for 'Maillard Reaction Products(MRPs)' made of sodium caseinate or whey protein isolates, and setting experiment for this. So I tried to dissolve MRPs ,which I thought it would be water-soluble, to distilled water with heating at 55oC, but it made protein precipitation. Also I dissolved it to DMSO and ethanol, but some of them still made precipitation in DMSO and all of them didn't dissolve in ethanol. What should I do?
Thank you for reading this and please let me know.
Probably the protein is denatured. It this difficult to guess, but I would try (a) a moderately basic media (casein and whey protein are much more soluble a high pH than at acidic or neutral pH); (b) A detergent. ionin (SDS) or non-ionic. It depends on what you want to do next, and possible interferences . Good luck
Considering its a protein-carbohydrate complex try Tris or Tween in pH 7.2 Phosphate Buffered Saline (PBS).
Hi Yujim Kim, if you still having problems maybe you have components with different polarity. Do you use an oil for your maillard reaction? for sure you have components solubles in water and anothers in oil. Firts, you should separate your components and then make an emulsion. For separation you can use a simple separation funnel and then for make an emulsion you can use tween. Add tween in the water part and then add the oil part slowly while mixing with a high-shear mixer (homogenizer).
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