How can I quantify F/G actin ratio in cells without kit?

12 December 2016 1 9K Report

Hi everyone,

Does anyone has a protocol that allows the extraction of F and G actin fraction in cells for the F:G actin ratio without using a kit? I have trouble to find that protocol but I know there must be some way to do it!

Thanks a lot!

Lee Roth

I asked about a kit, and got a non kit protocol here:

https://www.researchgate.net/post/Which_centrifuge_and_tubes_can_I_use_for_200ul_100_000g_37C_1h_Has_anyone_ever_worked_with_the_G-actin_F-actin_separation_kit_of_Cytoskeletoncom

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