02 February 2017 4 3K Report

Hello everyone,

I know it is a very basic question. Whenever I extract plasmid by alkaline lysis method and take nanodrop reading, it comes around 03-05ug/ul. Nanodrop peak is at exactly 260nm, 260/280 and 260/230 all within recommended range. But when I use it for digestion (e.g. 2-3ug) its intensity doesn't look like this. It seems faint as shown in attached gel picture.

Any suggestion or idea about this problem.

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