Best way to inactivate RNase III-A?

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Somatic mutations - how to validate NGS data?

Hi, I'm searching for methods to validate somatic mutations in pancreatic beta cells. The mutations are found by Next Generation Sequencing and I have try Sanger Sequencing with no results.

12 March 2018 1,821 5 View

Glucose stimulation of rat beta cells for insulin measurement?

Can anyone suggest a protocol for glucose stimulation of rat beta cells for insulin measurement. I can see final glucose concentration as 16,7mM but I dont know why?

26 February 2018 4,742 3 View

Normalization of small RNA-seq with housekeeping RNA?

I am working on some small RNA-Seq data and trying to normalize them. From literature, I found that people are using some housekeeping small RNA as reference for the normalization in qPCR. Can...

01 November 2016 4,047 3 View

What is production mechanism of Haft Kel field?

how is Haft Kel Field produced and how is the CO2 as injection considered in the flow simulation?

09 July 2016 882 2 View

Shelf life of TRIS?

I just started in a new lab and am about to start running western blot. However, the TRIS powder they have here is from 2002 and I'm a bit reluctant using it. Does anyone have experience using old...

26 April 2016 6,451 5 View

Does anyone know of an efficient, low-toxic caspase-1 inhibitor for in vitro cell culture?

I would like to inhibit the inflammasome in my cultured dendritic cells, but am a bit unsure of which one to buy. Does anyone have any recommendations? Thanks!

18 August 2013 2,847 2 View

Two questions: using fibronectin in transwell migration assays?

I've been having a lot of trouble trying to get my assays to work. I'm concerned that all I'm seeing is background migration. It's just B16BL6 melanoma cells (usually 1 x 10^5), using 10% FBS as a...

14 May 2013 5,684 15 View

Is possible to publish a phage draft genome as announcement in a journal (e.g. J virol or J Bact) or must you have the complete sequence?

What is the minimal genomic information to required to be accepted for publishing?

21 March 2012 4,643 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Tausif Alam

Do as it says...

Make the solution at desired concentration of RNAse in a good quality polypropylene tube that can sealed well. Place it in a boiling water bath and let it stand for 15-20 min. Remove the tube and let it cool down. Repeat the process one more time. Freeze the solution for storage and future use.