Best way to inactivate RNase III-A?

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Somatic mutations - how to validate NGS data?

Hi, I'm searching for methods to validate somatic mutations in pancreatic beta cells. The mutations are found by Next Generation Sequencing and I have try Sanger Sequencing with no results.

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Glucose stimulation of rat beta cells for insulin measurement?

Can anyone suggest a protocol for glucose stimulation of rat beta cells for insulin measurement. I can see final glucose concentration as 16,7mM but I dont know why?

26 February 2018 4,742 3 View

Normalization of small RNA-seq with housekeeping RNA?

I am working on some small RNA-Seq data and trying to normalize them. From literature, I found that people are using some housekeeping small RNA as reference for the normalization in qPCR. Can...

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What is production mechanism of Haft Kel field?

how is Haft Kel Field produced and how is the CO2 as injection considered in the flow simulation?

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Shelf life of TRIS?

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Does anyone know of an efficient, low-toxic caspase-1 inhibitor for in vitro cell culture?

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Two questions: using fibronectin in transwell migration assays?

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Is possible to publish a phage draft genome as announcement in a journal (e.g. J virol or J Bact) or must you have the complete sequence?

What is the minimal genomic information to required to be accepted for publishing?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

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How to repeat an operation on an atom selection for multiple times in PyMol?

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Michele Araújo Pereira

Hi Annette,

Is the RNase treatment for DNA extraction procedures?

Best,

Michele

Annette Turgeon

No, it's for WBC counting on Flow cytometry.

Tausif Alam

Do as it says...

Make the solution at desired concentration of RNAse in a good quality polypropylene tube that can sealed well. Place it in a boiling water bath and let it stand for 15-20 min. Remove the tube and let it cool down. Repeat the process one more time. Freeze the solution for storage and future use.