I intend to use casein micelles as nanovehicles for the delivery of a hydrophobic compound, by emulsifying the compound solution into a caseinate solution and then adding the salts to reassemble the micelles (According to Semo et al., 2007 and Zimet et al., 2011).
I know that I have to dilute my pure casein powder in a sodium hydroxide solution in order to obtain the sodium caseinate, but I'm not sure about the conditions to do that, such as NaOH concentration and temperature.
Can anybody help me with this, please?
Thanks
To best of my knowledge, the casein for its purification purpose is precipitated at its iso-electric point (pH 4.6). We can use the precipitate to dry it and use it as purified casein. However, to make the caseinate, we need to react it with sodium or ammonium hydroxide, then it becomes sodium or ammonium caseinate. I think that casein without a cation is not even called caseinate. In summary to make the caseinate we need to react it with sodium or ammonium hydroxide up to at least native pH (pH 6.8) of milk.
I hope it will help
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