I want to construct an shRNA-plasmid containing multi-domains of a single gene with one hairpin and under the control of single promoter. Is it possible?
No
Dear all I want to induce lymphoma in nude mice as an animal model.Can any one guide me before inoculation of related cell lines into nude mice which contamination biological agent such as...
06 July 2017 1,770 4 View
For confirmation the cloning of my insert (shRNA) in pRNAi vector, PCR did not respond at all, but when I tested the extracted plasmids of the same colonies with restricted enzyme (scaI), I found...
09 October 2013 1,435 3 View
I do not detect my insert in my colonies via colony pcr. What is the reason? I wanted to clone a shRNA in pRNAi. I digested my plasmid & pcr product of shRNA with the same RE. Then ligate them...
08 September 2013 5,266 1 View
When I digest the ends of the segment & the plasmid with restriction enzymes, they can not ligate together and although some times I have clones when I use colony PCR I do not have any band.
08 September 2013 2,480 7 View
I transfected a cell line with pRNAi vector and then added tetracyclin as an inducer of its promoter. How long do I have to wait if I want tetracyclin to induce the promoter of my plasmid and...
01 February 2013 5,708 3 View
My hela line grows very slowly. I changed my source and got new source of hela line from another institute, but when I passage this new source, the new flasks grow slowly again. what can I do...
31 December 2012 1,040 10 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View