I am teaching a undergraduate level Biochem Lab course. The first part of the labs are protocol-based experiments (protein extraction, purification, quantification and activity measurement). The remaining part will give students opportunities to put their topics of interest into their own mini projects, which will utilize the skills they have learned before. I will provide some topics for them as options if they don't have any ideas.
Here I am seeking any topics or experiments that are related to simple brain protein extraction and activity measurement. Does anyone have a mature protocol that fits in undergraduate level course?
In our teaching lab, we have sonicator, water bath, centrifuge, spectrometer, PCR, SDS gel apparatus, plate reader, Mass spec.
I will be appreciated if you share any of your ideas. Thank you.
Does it have to be from brains?
We routinely do purification of Lysozyme from egg white using CMC resin (let me know if you want a detailed protocol).
Biochemistry and Molecular Biology Education journal is a great place to start:
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1539-3429
For example:
Myoglobin from grounded meat
http://facstaff.bloomu.edu/pugh/BAMBED2002.pdf
GFP from recombinant E. coli using HIC
http://www.carolina.com/pdf/manuals/purification-green-fluor-protein-kit.pdf
Ferredoxin
http://www.unizar.es/departamentos/bioquimica_biologia/investigacion/mmedina/BesBAMBED.pdf
Invertase from yeasts
http://www.intechopen.com/books/protein-purification/the-isolation-of-invertase-from-baker-s-yeast-an-introduction-to-protein-purification-strategies
Hope this helps.
Yoram
Thank you very much, Yoram! I am the assist- instructor for the lab course. The principal instructor decided to use the old topic he used before for this time. But I think the ones you recommend are very interesting. I will let the principal instructor of the course know and we may try one of these for the next round! Thanks again for the useful information.
Best,
Lihui
If you want the Egg white Lysozyme purification protocol let me know, it is almost foolproof...
HI Yoram,
Could you share the eggwhite lysozyme protocol with me? Trying to setup a lab for an undergrad class in biochem.
Thanks,
Sravanti
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