Can anybody suggest an in-vitro method to analyse the microtubule depolymerisation?

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I need MD simulation in 500 ns for some of my test compounds with PBSA, GBSA and PCA. If a researcher will help, we will provide authorship?

For my research, I need MD simulation of 500 ns with PCA. Please reply if you have facility. We will provide corresponding authorship. The article will be published in a high IF journal. My email-...

26 November 2023 6,926 0 View

How to increase the solubility of chlorophyll extracted from plants for bioimaging studies?

Please provide information related to any coating that can be done or any composites that can be synthesized.

09 October 2023 9,003 2 View

Which families of freshwater fishes belong to 'True hill stream' fish families?

In the freshwater fish groups, some of the genera like Garra, Balitora, Labeo, Scizothorax etc are considered as true hill stream forms. On what basis this classification is considered?

02 August 2023 6,389 0 View

As we all know that Si 2p peak can be deconvoluted into two peaks such as Si 2p1/2 and Si 2p3/2. Which peak has higher binding energy and why ? ?

In the few literature, it was mentioned that the binding energy of Si 2p1/2 was higher than Si 2p3/2, while some authors made the just opposite statement about these peaks. I am so confused about...

22 June 2023 1,135 2 View

What is the JCPDS No and CCDC no for Ag-MOF-5?

Ag-MOF-5 synthesized from Terepthalic acid, Silver Nitrate and DMF. Need to know Chemical formula, Molecular weight and molecular structure of Ag-MOF-5. Thank you

21 May 2023 1,624 0 View

How to normalize total protein in Ponceau S stain?

I used to normalize my target protein using housekeeping genes. But now I have come to know about total protein normalization. So I would like to do the same. I want to know how to measure total...

11 March 2023 9,482 2 View

Can a composite show different dual phases (space group) in room temperature XRD and room temperature Neutron diffraction Reitveld refinement?

If possible kindly suggest some references.

05 February 2023 3,683 3 View

How to isolate fibroblast cells from hamster?

I isolated fibroblast cells from hamster by collagenase A digestion method. I used DMEM with extra L-glutamine as culture media. After 24hr there is no attachment of cells. The flask was coated...

28 November 2022 4,816 1 View

Which is the best technique- FACS or MACS for separation of dead cell from live cell population by using PI and SYTO9 fluorescence dye?

I want to separate dead cells from a bacterial population after treatment of any drug or antimicrobial agent. Which technique will be more efficient FACS or MACS if I use PI and SYTO9 dye ? Is...

15 August 2022 4,009 3 View

How to separate my metal microcage synthesised in ethylene glycol and water solvent ??

I tried to pellet it down at a maximum of 18K rpm, but it did not work. I heated the reaction mixture up to 200 C temp to evaporate the Ethylene Glycol(Including water) and to get the metal...

21 June 2022 6,740 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Sankalp Verma

Hi Saswat,

Could you be a little more specific related to what you want to study. As such depolymerization can be studied using dilution studies and cold treatment.

Rachel Elizabeth Moore

Hi Saswat, do you need help with experimental design or to analyse your results?

Saswat Mohapatra

Thanks to All

Dear Moore,I need some design regarding this..