What are the Biochemical methods for identifying Bifidobacterium and Lactobacillius colonies?
Hi
You can use EPI 50 kit, the biochemical tests was more and to need long time
Alaa Kareem Niamah Sir, is there any other laboratory method which can be performed without using kit.
Alaa Kareem Niamah Sir, is there any other laboratory method which can be performed without using kit.
Alaa Kareem Niamah Sir, can we identify it with any basic staining method or biochemical method?
No, you cann't staining method for identifition of lactic acid bacteria
you can selective media for idenifition of geuns only not speceis
Hello, If you want to differentiate lactobacillus and bifidobacteria, it is bit tricky but possible in lab. On basis of staining it is bit difficult but still lactobacillus will appear as Gram positive slender rods (big rods) mainly in chains or pairs. While bifidobacteria are pleomorphic in nature- so some may appear as coccoid or curved shaped within clusters or L-Y type arrangements.
Both are catalase negative. But sugar fermentation pattern can help you. Even analysis of metabolic end products can help to differentiate both as Lactobacillus , up to 90% lactic acid while bifidobactreia mainly produces acetic acid, so smell/ flavour gives indication in 10% skim milk after growth.
For major difference through biochemical tests, please refer Bergeys manual.
Best of luck!
Even growth media are different for both, MRS agar is selective for lactobacillus only. And even oxygen requirement for growth is also very different in both, most of bifidobacteria are strictly anaerobic in nature, while lactobacilus are aerotolerant, facultative in nature.
i am having some method for this.. but had to search in my pc.
vl reply you soon.
Hi sir Alaa Kareem Niamah, what is an EPI 50 kit? I could not search it in google.
you can grow Bifidobacterium on MRS agar in anaerobic chamber with gas kit, lactobacillus can grow without gas kit.
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