Hi,
I am not aware of the existence of such a website but I can share some of my own experience:
1. Use a kit if you can and a specialised plant kit is always better.
2. Don't let your sample thaw (it should always be flash frozen) before you add the buffer.
3. Don't keep unextracted samples for too long in a -80 freezer especially if you are planning an RNAseq, for qPCR the tolerance is a bit higher.
4. Good grinding of the sample is very important.
5. Phenol based extraction often interferes with your results.
6. Use gloves and sterile materials.
Good luck!
Dear Sanchari Kundu as Dr. Swetlana Boycheva Woltering mentioned earlier that some precautionary measures should be taken before RNA isolation. Firstly check RNA quality, purity, and yield before using it in several downstream applications.
(i) First, check RNA quality and purity using the RNA integrity number (RIN), and the A260/A280 ratio. If the sample's RNA quality is poor, this is probably the cause of poor results. In this case, either isolated RNA was degraded before the samples were processed or RNA samples were degraded during the entire RNA preparation. The RNA preparation should be repeated with freshly harvested samples and with more care to prevent RNase degradation or contamination during the entire isolation procedure.
(ii) If the quality of RNA is good, check the yield of isolated RNA. If the RNA quality is good but the yield is lower than expected, this might be due to incomplete cell lysis or might be poor binding, or poor elution.
(iii) The RNA preparation should be repeated with more care to make sure the sample is completely disrupted in the RNA lysis buffer. If the RNA yield is lower than expected, RNA may need to be concentrated, or even more RNA isolation needs to be done.
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