Yes, a phosphate buffer made from disodium hydrogen phosphate (Na2HPO4) and potassium dihydrogen phosphate (KH2PO4) can replace a normal sodium phosphate buffer for protein purification. In fact, phosphate buffers are commonly used in protein purification because they are effective at maintaining the pH of the solution and are compatible with many biological molecules.
Both sodium phosphate buffer and a buffer made from disodium hydrogen phosphate and potassium dihydrogen phosphate can be used to adjust the pH of the solution to different levels. The choice of buffer will depend on the specific needs of the protein purification process, such as the optimal pH range for the protein of interest and the compatibility of the buffer with other chemicals or reagents used in the purification process.
It is important to note that the concentration and pH of the buffer should be carefully controlled during the protein purification process to ensure optimal protein stability and activity. Additionally, the buffer should be properly prepared and stored to avoid contamination or changes in pH over time.
Thank you very much Karim for the explanatory answer added regarding this.
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