Samrina Ahmed

21 Questions 19 Answers 0 Followers

Questions related from Samrina Ahmed

Mangroves leaves area formula?

I want to estimate leaf area of Mangrove plant.For calculation i have seen same formula but different factor number(I think it is factor) like, A = L x W x 0.75. A = L x W x 0.55 so i need...

02 February 2019 8,619 1 View

Total Chlorophyll value higher in stress plants?

Hi all, Recently i estimated Chlorophyll in mangroves plant under 2 weeks of non-essential metal.My result is showed different attitude that as concentration higher,chlorophyll also higher( chl...

02 February 2019 4,464 4 View

Extinction coefficient required?

I measure Poly phenol Oxidase (PPO) by Mayer 1965.Now for calculation i required extinction coefficient for poly phenol oxidase? So please share. i have seen so many papers but did not find...

09 September 2018 5,190 0 View

I need help regarding calculation of antioxidant enzyme like catalase,Ascorbate peroxidase etc?

Dear all,I am stuck in calculation of CAT and APx activities in plants.I used SPECIFIC ENZYMATIC ACTIVITY formula.For CAT assay i used 100ul plant enzyme but for APx i used 40ul plant enzyme. So...

02 February 2018 9,273 4 View

Book name required?

For my enzymatic and non enzymatic antioxidant like Catalase,peroxidase,Glutathione etc in plants required any book(online PDF or purchase) in which I get all information +Assay protocol...

02 February 2018 6,893 0 View

Research paper required?

Heloo every one! I need help regarding to research paper/proposal on Long term abiotic stress on antioxidant activity in plants in different time interval. Please share

11 November 2017 8,492 4 View

Required Method in Enzomology book pdf.

i am again submitting my question regarding pdf or any form of documents of book.(I think my question has omitted from this page) Method in Enzymology volume 234 page # 270-285. I google it but...

08 August 2017 5,961 2 View

Standard value increase or decrease?

Heloo All.I am making H2O2 standard graph. My min.value (O.D)of Std.curve is 0.241and max.(O.D)is 0.349.where as my samples O.D (absorbance of spectrophotometer)are below then 0.241.so can I use...

08 August 2017 2,012 0 View

How can i calculate dilution factor(df)in my sample?

I have taken 2 mg leaves and crush in 5 ml solution.Actually i have to take 2 mg leave sample and 2 ml solution. So during calculation how can i calculate df in my formula. Smilarly if i add my...

07 July 2017 8,067 4 View

Is any one guide me how to calculate antioxidant activity?

Dear all,i want to calculate antioxidant activity (APX,GR,GPX,CAT,PPO)by this " μmol min-1 g-1 FW". Please by any example guide me.

03 March 2017 4,065 9 View

Please share papers related to antioxidant?

I want to write up my thesis so need research paper related to **Effect of A-biotic stress on enzymatic and non enzymatic antioxidant level in plants** If you give any guide line related to write...

02 February 2017 8,875 2 View

Dear All.I need review paper of Plant responses against Heavy metal stress?

I am searching Newly published review paper about Plant tolerance mechanism against heavy metal stress /toxicity.If any one can help i will be appreciated.

12 December 2016 3,889 4 View

Does anyone have a protocol of Glutathione Peroxidase?

I need detailed protocol assay of Glutathione Peroxidase of Rotruck et al 1973.And how to calculate absorbance.with reference

08 August 2016 7,095 1 View

How long can we store plant extracts ( solvent extracted ) before using for antioxident?

I want to perform Reduced Glutathione,thiol and Lipid peroxidation.so if i extracted my plant sample, How long will the bioactive compounds show activity if it is stored in -80 OC?

04 April 2016 1,522 11 View

In Peroxidase (POD) protocol by Reddy et al 1995, what does absorbance change mean?

Hello Researchers.In protocol of POD it is written that change in absorbance/minute at 430 nm. I just want to ask that absorbance become increase or decrease in stress plant sample? Also guide me...

01 January 2016 3,583 4 View

Does any one tell me about method of GLUTATHIONE S-TRANSFERASE (GST)?

I have a method of Habig et al. (1974).but some of chemical quantity is not clear,like how can i make solution of Glutothione(1mM)?also CDNB (1mM in ethanol.how i prepare.please guide me and send...

01 January 2015 2,508 1 View

Is their any method of estimation of oxidative enzyme and non-oxidative enzyme work on ELISA reader?

If i am taking reading from Elasa and Spectrophotometric,so which types of changes appear in reading?Like catalase.So can i do my research work on ELISA or not?

12 December 2014 581 3 View

How can I remove debris from supernatant of extract?

I have extract of roots and leave of Mangrove,but after centrifuge some of debris float in superintendent,so how can i remove it.Extract are just 1-1.5 ml so not possible to filter.Please guide

12 December 2014 9,766 4 View

Is there any method of Superoxide Dismutase (SOD),where hydroxyl amine hydrochloride is used?

I need that method where hydroxyl amine hydrocloride is used for plants.

11 November 2014 2,587 7 View

Which type of precautions are required for carrying different parts of plants from the greenhouse to the lab?

I am working on enzyme in plants, so when I plug leaves etc then how will I carry from greenhouse to lab (Too far )? Is there any tip?

10 October 2014 7,411 3 View

How can I prepare a sodium pyrophosphate buffer 0.025M and pH 8.3?

I need a procedure.

10 October 2014 1,456 1 View