08 August 2016 1 7K Report

I need detailed protocol assay of Glutathione Peroxidase of Rotruck et al 1973.And how to calculate absorbance.with reference

More Samrina Ahmed's questions See All
Total Chlorophyll value higher in stress plants?

Hi all, Recently i estimated Chlorophyll in mangroves plant under 2 weeks of non-essential metal.My result is showed different attitude that as concentration higher,chlorophyll also higher( chl...

01 February 2019 4,486 4 View

Mangroves leaves area formula?

I want to estimate leaf area of Mangrove plant.For calculation i have seen same formula but different factor number(I think it is factor) like, A = L x W x 0.75. A = L x W x 0.55 so i need...

01 February 2019 8,641 1 View

Extinction coefficient required?

I measure Poly phenol Oxidase (PPO) by Mayer 1965.Now for calculation i required extinction coefficient for poly phenol oxidase? So please share. i have seen so many papers but did not find...

08 September 2018 5,215 0 View

Book name required?

For my enzymatic and non enzymatic antioxidant like Catalase,peroxidase,Glutathione etc in plants required any book(online PDF or purchase) in which I get all information +Assay protocol...

01 February 2018 6,916 0 View

I need help regarding calculation of antioxidant enzyme like catalase,Ascorbate peroxidase etc?

Dear all,I am stuck in calculation of CAT and APx activities in plants.I used SPECIFIC ENZYMATIC ACTIVITY formula.For CAT assay i used 100ul plant enzyme but for APx i used 40ul plant enzyme. So...

01 February 2018 9,293 4 View

Research paper required?

Heloo every one! I need help regarding to research paper/proposal on Long term abiotic stress on antioxidant activity in plants in different time interval. Please share

10 November 2017 8,519 4 View

Required Method in Enzomology book pdf.

i am again submitting my question regarding pdf or any form of documents of book.(I think my question has omitted from this page) Method in Enzymology volume 234 page # 270-285. I google it but...

07 August 2017 5,987 2 View

Standard value increase or decrease?

Heloo All.I am making H2O2 standard graph. My min.value (O.D)of Std.curve is 0.241and max.(O.D)is 0.349.where as my samples O.D (absorbance of spectrophotometer)are below then 0.241.so can I use...

07 August 2017 2,035 0 View

How can i calculate dilution factor(df)in my sample?

I have taken 2 mg leaves and crush in 5 ml solution.Actually i have to take 2 mg leave sample and 2 ml solution. So during calculation how can i calculate df in my formula. Smilarly if i add my...

06 July 2017 8,089 4 View

Is any one guide me how to calculate antioxidant activity?

Dear all,i want to calculate antioxidant activity (APX,GR,GPX,CAT,PPO)by this  " μmol min-1 g-1 FW". Please by any example guide me.

02 March 2017 4,092 9 View

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