I have a method of Habig et al. (1974).but some of chemical quantity is not clear,like how can i make solution of Glutothione(1mM)?also CDNB (1mM in ethanol.how i prepare.please guide me and send me detail methodology?
hello i have a protocol i will send you.
this is the same protocol i have used for my work.
best luck
Hi all, Recently i estimated Chlorophyll in mangroves plant under 2 weeks of non-essential metal.My result is showed different attitude that as concentration higher,chlorophyll also higher( chl...
01 February 2019 4,407 4 View
I want to estimate leaf area of Mangrove plant.For calculation i have seen same formula but different factor number(I think it is factor) like, A = L x W x 0.75. A = L x W x 0.55 so i need...
01 February 2019 8,571 1 View
I measure Poly phenol Oxidase (PPO) by Mayer 1965.Now for calculation i required extinction coefficient for poly phenol oxidase? So please share. i have seen so many papers but did not find...
08 September 2018 5,140 0 View
For my enzymatic and non enzymatic antioxidant like Catalase,peroxidase,Glutathione etc in plants required any book(online PDF or purchase) in which I get all information +Assay protocol...
01 February 2018 6,839 0 View
Dear all,I am stuck in calculation of CAT and APx activities in plants.I used SPECIFIC ENZYMATIC ACTIVITY formula.For CAT assay i used 100ul plant enzyme but for APx i used 40ul plant enzyme. So...
01 February 2018 9,220 4 View
Heloo every one! I need help regarding to research paper/proposal on Long term abiotic stress on antioxidant activity in plants in different time interval. Please share
10 November 2017 8,425 4 View
i am again submitting my question regarding pdf or any form of documents of book.(I think my question has omitted from this page) Method in Enzymology volume 234 page # 270-285. I google it but...
07 August 2017 5,911 2 View
Heloo All.I am making H2O2 standard graph. My min.value (O.D)of Std.curve is 0.241and max.(O.D)is 0.349.where as my samples O.D (absorbance of spectrophotometer)are below then 0.241.so can I use...
07 August 2017 1,956 0 View
I have taken 2 mg leaves and crush in 5 ml solution.Actually i have to take 2 mg leave sample and 2 ml solution. So during calculation how can i calculate df in my formula. Smilarly if i add my...
06 July 2017 8,015 4 View
02 March 2017 4,017 9 View
Please provide Book Title and author name
02 March 2021 9,059 3 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
01 March 2021 3,905 5 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...
26 February 2021 6,905 10 View
I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...
24 February 2021 2,265 3 View
For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...
23 February 2021 1,346 13 View
I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .
22 February 2021 1,131 4 View
Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...
21 February 2021 4,634 6 View
Please suggest ways to learn Fuzzy VIKOR methodology in a best possible way. And is there any toolkit available for the same? Thank you in advance.
20 February 2021 8,271 3 View