Total Chlorophyll value higher in stress plants?

Hi all, Recently i estimated Chlorophyll in mangroves plant under 2 weeks of non-essential metal.My result is showed different attitude that as concentration higher,chlorophyll also higher( chl...

01 February 2019 4,407 4 View

Mangroves leaves area formula?

I want to estimate leaf area of Mangrove plant.For calculation i have seen same formula but different factor number(I think it is factor) like, A = L x W x 0.75. A = L x W x 0.55 so i need...

01 February 2019 8,571 1 View

Extinction coefficient required?

I measure Poly phenol Oxidase (PPO) by Mayer 1965.Now for calculation i required extinction coefficient for poly phenol oxidase? So please share. i have seen so many papers but did not find...

08 September 2018 5,140 0 View

Book name required?

For my enzymatic and non enzymatic antioxidant like Catalase,peroxidase,Glutathione etc in plants required any book(online PDF or purchase) in which I get all information +Assay protocol...

01 February 2018 6,839 0 View

I need help regarding calculation of antioxidant enzyme like catalase,Ascorbate peroxidase etc?

Dear all,I am stuck in calculation of CAT and APx activities in plants.I used SPECIFIC ENZYMATIC ACTIVITY formula.For CAT assay i used 100ul plant enzyme but for APx i used 40ul plant enzyme. So...

01 February 2018 9,220 4 View

Research paper required?

Heloo every one! I need help regarding to research paper/proposal on Long term abiotic stress on antioxidant activity in plants in different time interval. Please share

10 November 2017 8,425 4 View

Required Method in Enzomology book pdf.

i am again submitting my question regarding pdf or any form of documents of book.(I think my question has omitted from this page) Method in Enzymology volume 234 page # 270-285. I google it but...

07 August 2017 5,911 2 View

Standard value increase or decrease?

Heloo All.I am making H2O2 standard graph. My min.value (O.D)of Std.curve is 0.241and max.(O.D)is 0.349.where as my samples O.D (absorbance of spectrophotometer)are below then 0.241.so can I use...

07 August 2017 1,956 0 View

How can i calculate dilution factor(df)in my sample?

I have taken 2 mg leaves and crush in 5 ml solution.Actually i have to take 2 mg leave sample and 2 ml solution. So during calculation how can i calculate df in my formula. Smilarly if i add my...

06 July 2017 8,015 4 View

Is any one guide me how to calculate antioxidant activity?

02 March 2017 4,017 9 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View