I need a procedure.
@ Peter,
For Sigma-Aldrich P-8910, sodium pyrophosphate tetrabasic M.W = 265.90 [http://www.sigmaaldrich.com/catalog/product/aldrich/p8010?lang=en®ion=US]
So, one should dissolve 0.66 grams in 100 mL water to reach 0.025M solution (265.90 x 0.1 x 0.025 = 0.66 grams.)
Hi all, Recently i estimated Chlorophyll in mangroves plant under 2 weeks of non-essential metal.My result is showed different attitude that as concentration higher,chlorophyll also higher( chl...
01 February 2019 4,486 4 View
I want to estimate leaf area of Mangrove plant.For calculation i have seen same formula but different factor number(I think it is factor) like, A = L x W x 0.75. A = L x W x 0.55 so i need...
01 February 2019 8,641 1 View
I measure Poly phenol Oxidase (PPO) by Mayer 1965.Now for calculation i required extinction coefficient for poly phenol oxidase? So please share. i have seen so many papers but did not find...
08 September 2018 5,215 0 View
For my enzymatic and non enzymatic antioxidant like Catalase,peroxidase,Glutathione etc in plants required any book(online PDF or purchase) in which I get all information +Assay protocol...
01 February 2018 6,916 0 View
Dear all,I am stuck in calculation of CAT and APx activities in plants.I used SPECIFIC ENZYMATIC ACTIVITY formula.For CAT assay i used 100ul plant enzyme but for APx i used 40ul plant enzyme. So...
01 February 2018 9,293 4 View
Heloo every one! I need help regarding to research paper/proposal on Long term abiotic stress on antioxidant activity in plants in different time interval. Please share
10 November 2017 8,519 4 View
i am again submitting my question regarding pdf or any form of documents of book.(I think my question has omitted from this page) Method in Enzymology volume 234 page # 270-285. I google it but...
07 August 2017 5,987 2 View
Heloo All.I am making H2O2 standard graph. My min.value (O.D)of Std.curve is 0.241and max.(O.D)is 0.349.where as my samples O.D (absorbance of spectrophotometer)are below then 0.241.so can I use...
07 August 2017 2,035 0 View
I have taken 2 mg leaves and crush in 5 ml solution.Actually i have to take 2 mg leave sample and 2 ml solution. So during calculation how can i calculate df in my formula. Smilarly if i add my...
06 July 2017 8,089 4 View
Dear all,i want to calculate antioxidant activity (APX,GR,GPX,CAT,PPO)by this " μmol min-1 g-1 FW". Please by any example guide me.
02 March 2017 4,092 9 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View