Total Chlorophyll value higher in stress plants?

Hi all, Recently i estimated Chlorophyll in mangroves plant under 2 weeks of non-essential metal.My result is showed different attitude that as concentration higher,chlorophyll also higher( chl...

01 February 2019 4,407 4 View

Mangroves leaves area formula?

I want to estimate leaf area of Mangrove plant.For calculation i have seen same formula but different factor number(I think it is factor) like, A = L x W x 0.75. A = L x W x 0.55 so i need...

01 February 2019 8,571 1 View

Extinction coefficient required?

I measure Poly phenol Oxidase (PPO) by Mayer 1965.Now for calculation i required extinction coefficient for poly phenol oxidase? So please share. i have seen so many papers but did not find...

08 September 2018 5,140 0 View

I need help regarding calculation of antioxidant enzyme like catalase,Ascorbate peroxidase etc?

Dear all,I am stuck in calculation of CAT and APx activities in plants.I used SPECIFIC ENZYMATIC ACTIVITY formula.For CAT assay i used 100ul plant enzyme but for APx i used 40ul plant enzyme. So...

01 February 2018 9,220 4 View

Research paper required?

Heloo every one! I need help regarding to research paper/proposal on Long term abiotic stress on antioxidant activity in plants in different time interval. Please share

10 November 2017 8,425 4 View

Required Method in Enzomology book pdf.

i am again submitting my question regarding pdf or any form of documents of book.(I think my question has omitted from this page) Method in Enzymology volume 234 page # 270-285. I google it but...

07 August 2017 5,911 2 View

Standard value increase or decrease?

Heloo All.I am making H2O2 standard graph. My min.value (O.D)of Std.curve is 0.241and max.(O.D)is 0.349.where as my samples O.D (absorbance of spectrophotometer)are below then 0.241.so can I use...

07 August 2017 1,956 0 View

How can i calculate dilution factor(df)in my sample?

I have taken 2 mg leaves and crush in 5 ml solution.Actually i have to take 2 mg leave sample and 2 ml solution. So during calculation how can i calculate df in my formula. Smilarly if i add my...

06 July 2017 8,015 4 View

Is any one guide me how to calculate antioxidant activity?

02 March 2017 4,017 9 View

Please share papers related to antioxidant?

01 February 2017 8,825 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View