Dear all,i want to calculate antioxidant activity (APX,GR,GPX,CAT,PPO)by this " μmol min-1 g-1 FW".
Please by any example guide me.
hello,
for the time you just have to mesure the time during 2 mesurements and divise the result by this times in minuts. For the fresh weight you just have to weight your sample first.
To put the result in μmol it depend if you want it by μmol of enzyme, or by the μmol of product prodiuce by the reaction. In the two cases you need product do make a standard curve
This the mentioned thesis that measuring the number of antioxidants activity I think It was very beneficial for you.
This dear samera.
Thesis Correlation of Leptin and Resistin with Oxidative Stress in ...
This book chapter describes in detail how to perform the assays and calculations:
Regoli, F., Bocchetti, R. and Wilhelm Filho, D. (2011). Spectrophotometric assays of antioxidants. In Oxidative Stress in Aquatic Ecosystems (eds. Abele, D., Vázquez-Medina, J. P., and Zenteno-Savín, T., pp. 367–380. Chichester, UK: John Wiley & Sons, Ltd.
http://onlinelibrary.wiley.com/doi/10.1002/9781444345988.ch27/summary
Dear Samrina
Do you mean to measure the antioxidant enzyme activities or antioxidant activity of some extract or compound??
Dear,
Samrina Ahmed you may calculate activities of antioxidant enzymes using extinction coefficient for this you may go through the following attached book.
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