I want to perform Reduced Glutathione,thiol and Lipid peroxidation.so if i extracted my plant sample, How long will the bioactive compounds show activity if it is stored in -80 OC?
Not more than two days,but the best method of storage is refrigeration. This can keep the bioactive compound for more a week.
There should be no problem with the storage conditions you have. The extracts will remain stable for a long time but the sooner the better. If you have all the facilities, perform the assay with the fresh extract once. Later you can also compare the results with the stored extracts. This will also allow you to answer your question by comparing the two results and the difference found, if any.
It depends how active against free radicals are Your samples. Normally -80 C should keep the samples safe for a couple of days but remember that even in this very low temperature the atmosfere is still with oxygen so there is some probability that Your samples will lose some of their antioxidant activity. The best way is to perform antioxidant assays on fresh extracts. The sooner the better. But in practice there should not be any problem during one week period of time.
Good luck
It depends on the active constituent of the plant under investigation. Antioxidant phytochemicals such as tetraterpen are more stable than phenolic antioxidant.
In general freezing is the best method for storage of plant extract
you can store this in refrigerator for one week to month. But its better to perform the analysis as soon as possible.
Its depend on your plant extract character. But my suggestion is if you want desired result its better to perform the experiment.
thanks you all.Sir so if I make powder(fresh leave and root) with the help of liquid nitrogen,then can i store my sample so long (1 year at least) for antioxidant analysis ,Because after powder cell wall and cell membrane and organelles become damage?
Hi you need to lyophilized if an aqueous extract and to reduce the organic solvent in your extract.
Antioxidant properties of plant extract are generally due to the phenolic components which are actively involved in the redox reaction and scavenge the radicals. In principle, if you do not add any redox active solvent or do not expose your extract to highly oxidising conditions, their capacity of being antioxidant is retained. It is therefore necessary to store such compounds in sealed (airtight) containers and away from the light (many of the antioxidants are photo active and may undergo photo-catalysed oxidation). As suggested in aforesaid responses it is advised to store them in refrigeration to protect biological compartments, which will definitely protect the intracellular cellular environment and therefore increase the shelf like. Dried tea leaves which are transported and sold even after a year retain their antioxidant activity. IF Not their benefits as antioxidant would have diminished.
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