26 Questions 16 Answers 0 Followers
Questions related from Raksha Bawankar
Is it possible to find out the integrity of DNA using real time PCR for comparison among two different species?
03 March 2018 9,581 4 View
The data sheet provided by company has mentioned that dissolve the primer in 200ul of sterile water or TE and it gives 242.65pm. How to prepare the working stock? Which concentration of the...
04 April 2017 9,149 4 View
I need to perform inverse PCR, is their any software available to design primers for inverse PCR? Thank You
01 January 2016 7,470 1 View
If we need to overexpress particular gene in pCambia 1301, do we need to do any changes in gene first before performing cloning. Thank You Raksha
08 August 2014 340 9 View
To perform a native PAGE assay for peroxidase, what can be the chemical used for its staining?
05 May 2014 4,633 5 View
What is the best percentage of glycerol stock preparation for transformed culture is recommended for the long term storage? How long will cultures be efficiency maintained?
04 April 2014 3,380 26 View
How much efficiency will remain in commercially available competent cells after one month?
12 December 2013 2,071 13 View
To get good phylogenetic tree I need to get some sequences trimmed. I have searched for it but was unable to perform.
12 December 2013 6,399 2 View
I got the sequence from the database in the FASTA format. I need to find the CDS region from the same. The sequence is from KEGG pathway database.
11 November 2013 7,348 10 View
If a vector map is given two antibiotics and we will be growing it in only one antibiotic what are the possibilities to lose a plasmid?
10 October 2013 9,458 1 View
Can we use absolute methanol in the soxlet apparatus. And what is the best temperature for its use?
10 October 2013 2,707 15 View
What is the good hormone for an explant from tomato?
09 September 2013 1,465 5 View
I am facing some problem while using this qiagen tips
07 July 2013 1,674 10 View
Regarding Real Time PCR
07 July 2013 273 11 View
How many milligrams of sample is recommended for HPLC and GC MS analysis?
07 July 2013 6,894 5 View
If the database of a particular plant with a specific gene is not available on NCBI, what is the best way to do a BLAST of the received sequence after sequencing?
06 June 2013 6,931 3 View
What solvent will be best for extract preparation and which will give good results for stigmasterol estimation? The tissue is part of a tomato plant.
05 May 2013 3,213 15 View
I have the data for a gene network obtained from HumanNet but I am unable to interpret the scores been given from HumanNet. I would like to know on what basis these scores have been calculated....
01 January 2013 8,036 2 View
I need to perform HPLC analysis at different growth stages of plant. So can I prepare extract from fresh tissue itself.
01 January 2013 4,732 48 View
I am working with pathways. I have sequence for 7 different genes in 6 different plants. Now I want to perform phylogenetic analysis for individual genes in six different plants. Do you have any...
10 October 2012 3,555 61 View
What protocol do you follow to get the best results? Since I am new to this technique, in this experiment I have some difficulties with the results.
09 September 2012 8,670 0 View
What protocol do you follow to get the best results? Since I am new to this technique, in this experiment I have some difficulties with the results. Thanks in advance .
08 August 2012 6,827 4 View
Can anyone suggest a software to identify the introns and exons present in a sequence? The sequence is not available in NCBI.
08 August 2012 7,904 16 View
We have grown Arabidopsis plants in our laboratory within the required growth conditions. But, maybe due to the lack of proper growth conditions, they are not showing good growth. I need to...
03 March 2012 4,274 6 View
I need to perform molecular biology experiments on Arabidopsis plant leaf. Can anyone suggest which would be the easiest method to get a good number of plants from seeds?
08 August 2011 3,716 6 View
If we need to perform molecular work with leaves of Arabidopsis plant, what will be the best method to grow Arabidopsis seeds?
08 August 2011 6,820 7 View
