Is their any alternative to benzidine chemicals for the staining of peroxidase native PAGE?

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Nitesh S. Litoriya

hii Raksha,

when i did peroxidase, i used hydrogen peroxide, followed by ortho-dianisidine dye in phosphate buffer.

Deepti Agrawal

Hey raksha, nitesh has rigthly quoted. Here is a paper for your reference further

Raksha Bawankar

Thanxs a lot to both of you for your valuable reply. It may help me.

Francisco Solano

Benzidine and o-dianisidine oxidation products are quite soluble, so the bands tend to dissapear after some time. 3,3'-diaminobenzidine and methylbenzidine derivatives (TMB) yield more insoluble products, and the bands are more stable.

Anil Kumar

You can use o-dianisidine in place of benzidine