What protocol do you follow to get the best results? Since I am new to this technique, in this experiment I have some difficulties with the results.
Is it possible to find out the integrity of DNA using real time PCR for comparison among two different species?
02 March 2018 9,608 4 View
The data sheet provided by company has mentioned that dissolve the primer in 200ul of sterile water or TE and it gives 242.65pm. How to prepare the working stock? Which concentration of the...
03 April 2017 9,186 4 View
I need to perform inverse PCR, is their any software available to design primers for inverse PCR? Thank You
31 December 2015 7,494 1 View
If we need to overexpress particular gene in pCambia 1301, do we need to do any changes in gene first before performing cloning. Thank You Raksha
07 August 2014 367 9 View
To perform a native PAGE assay for peroxidase, what can be the chemical used for its staining?
04 May 2014 4,661 5 View
What is the best percentage of glycerol stock preparation for transformed culture is recommended for the long term storage? How long will cultures be efficiency maintained?
03 April 2014 3,413 26 View
How much efficiency will remain in commercially available competent cells after one month?
11 December 2013 2,107 13 View
To get good phylogenetic tree I need to get some sequences trimmed. I have searched for it but was unable to perform.
11 December 2013 6,434 2 View
I got the sequence from the database in the FASTA format. I need to find the CDS region from the same. The sequence is from KEGG pathway database.
10 November 2013 7,379 10 View
If a vector map is given two antibiotics and we will be growing it in only one antibiotic what are the possibilities to lose a plasmid?
09 October 2013 9,501 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...
11 July 2024 4,284 0 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
11 July 2024 9,010 3 View
This question seeks to address the growing concern of cloned academic journals, which are fraudulent duplicates of legitimate publications. It aims to guide researchers, scholars, and academics on...
10 July 2024 5,966 2 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View