How to find out the integrity of DNA after performing Real Time PCR?

Is it possible to find out the integrity of DNA using real time PCR for comparison among two different species?

02 March 2018 9,554 4 View

How to prepare the working stock if the primer concentration in 242.65pm/ul on addition of 200ul water?

03 April 2017 9,122 4 View

What are the important steps to perform overexpression of plant gene in pCambia 1301?

If we need to overexpress particular gene in pCambia 1301, do we need to do any changes in gene first before performing cloning. Thank You Raksha

07 August 2014 312 9 View

Is their any alternative to benzidine chemicals for the staining of peroxidase native PAGE?

To perform a native PAGE assay for peroxidase, what can be the chemical used for its staining?

04 May 2014 4,607 5 View

What is the best percentage of glycerol stock preparation for transformed culture recommended for the long term storage?

What is the best percentage of glycerol stock preparation for transformed culture is recommended for the long term storage? How long will cultures be efficiency maintained?

03 April 2014 3,349 26 View

To what extent is it advisable to use commercially available competent cells for transformation?

How much efficiency will remain in commercially available competent cells after one month?

11 December 2013 2,046 13 View

For Coding sequence trimming which online tool should be used?

To get good phylogenetic tree I need to get some sequences trimmed. I have searched for it but was unable to perform.

11 December 2013 6,373 2 View

Can anyone suggest me how to find out the CDS region from the FASTA sequence?

I got the sequence from the database in the FASTA format. I need to find the CDS region from the same. The sequence is from KEGG pathway database.

10 November 2013 7,317 10 View

How probable is the possibility of losing a vector culture if we grow it in only one antibiotic?

If a vector map is given two antibiotics and we will be growing it in only one antibiotic what are the possibilities to lose a plasmid?

09 October 2013 9,422 1 View

What is the best percentage of methanol to use in soxlet apparatus?

Can we use absolute methanol in the soxlet apparatus. And what is the best temperature for its use?

09 October 2013 2,680 15 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View