Is their any software available to design primer for inverse PCR?

Raksha Bawankar @Raksha_Bawankar

01 January 2016 1 8K Report

I need to perform inverse PCR, is their any software available to design primers for inverse PCR?

Thank You

Raksha Bawankar

Dear sir

Thanks a lot for your reply. I will try this.

Badges
Science method

More Raksha Bawankar's questions See All

How to find out the integrity of DNA after performing Real Time PCR?

Is it possible to find out the integrity of DNA using real time PCR for comparison among two different species?

02 March 2018 9,608 4 View

How to prepare the working stock if the primer concentration in 242.65pm/ul on addition of 200ul water?

The data sheet provided by company has mentioned that dissolve the primer in 200ul of sterile water or TE and it gives 242.65pm. How to prepare the working stock? Which concentration of the...

03 April 2017 9,186 4 View

What are the important steps to perform overexpression of plant gene in pCambia 1301?

If we need to overexpress particular gene in pCambia 1301, do we need to do any changes in gene first before performing cloning. Thank You Raksha

07 August 2014 367 9 View

Is their any alternative to benzidine chemicals for the staining of peroxidase native PAGE?

To perform a native PAGE assay for peroxidase, what can be the chemical used for its staining?

04 May 2014 4,661 5 View

What is the best percentage of glycerol stock preparation for transformed culture recommended for the long term storage?

What is the best percentage of glycerol stock preparation for transformed culture is recommended for the long term storage? How long will cultures be efficiency maintained?

03 April 2014 3,413 26 View

To what extent is it advisable to use commercially available competent cells for transformation?

How much efficiency will remain in commercially available competent cells after one month?

11 December 2013 2,107 13 View

For Coding sequence trimming which online tool should be used?

To get good phylogenetic tree I need to get some sequences trimmed. I have searched for it but was unable to perform.

11 December 2013 6,434 2 View

Can anyone suggest me how to find out the CDS region from the FASTA sequence?

I got the sequence from the database in the FASTA format. I need to find the CDS region from the same. The sequence is from KEGG pathway database.

10 November 2013 7,379 10 View

How probable is the possibility of losing a vector culture if we grow it in only one antibiotic?

If a vector map is given two antibiotics and we will be growing it in only one antibiotic what are the possibilities to lose a plasmid?

09 October 2013 9,501 1 View

What is the best percentage of methanol to use in soxlet apparatus?

Can we use absolute methanol in the soxlet apparatus. And what is the best temperature for its use?

09 October 2013 2,748 15 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View